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Cysteine peptidases of Eudiplozoon nipponicum: a broad repertoire of structurally assorted cathepsins L in contrast to the scarcity of cathepsins B in an invasive species of haematophagous monogenean of common carp

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Figure

Fig. 1 Relative transcription rate of E. nipponicum cathepsins L. Therelative transcription rate predicted by a back mapping of raw Illuminareads to the assembled transcripts was calculated as the percentage ofall cathepsin L transcripts
Fig. 3 SDS-PAGE and affinityyrEnCL3−/immunoblots of yrEnCL1, yrEnCL3 andyrEnCB after purification
Fig. 4 SDS-PAGE and active site labelling of purifiedprocessed to a 28 kDa form during incubation in 50 mM/100 mM CPB containing 2 mM DTT at 37 °C
Fig. 6 SDS-PAGE of protein substrates degraded by recombinantalbumin.Lane 2:the enzymes were incubated overnight with various protein substrates in 50 mM/100 mM CPB pH 4.5 containing 2 mM DTT
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