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Characterization of a secreted cystatin of the parasitic nematode Haemonchus contortus and its immune modulatory effect on goat monocytes

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Figure

Fig. 1 Putative amino acid alignment of HCcyst-3 with another nematode type 2 cystatins: CEcyst-1 of Caenorhabditis elegans(GenBank: AF100663);CEcyst-2 of Caenorhabditis elegans(GenBank: AF068718); NBcyst of Nippostrongylus brasiliensis(GenBank: AB050883);
Fig. 3 Immunohistochemical localization of the HCcyst-3 protein in a cryostal section ofimmunofluorescence method using second antibody Cy3 labeled goat anti-rat IgG (ab6953, Abcam)
Table 1 Protease inhibition assays
Fig. 5 Uptake of rHCcyst-3 by goat monocytes. Goat monocytes were left untreated or incubated with rHCcyst-3 (40All cells were fixed and incubated with rat anti-rHCcyst-3 antibody followed by Cy3 labeled goat anti-rat IgG (red)
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