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Adenovirus Vectors with the 100K Gene Deleted and Their Potential for Multiple Gene Therapy Applications

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Figure

FIG. 1. (A) Cytopathic effects in infected cells. The mutant virus H5tspermissive temperature of 32°C or the nonpermissive temperature of 39°C
FIG. 2. (A) Assembly of [100K�combination in bacterial plasmids. Construction of [E1AdCMVscreen kanamycin-resistant colonies for identification of clones con-taining recombinant pAd[E1linearize the p[E1the Ad inverted terminal repeat elements; step 4, trans
FIG. 3. Growth of modified vectors only in 100K-transcomple-menting cell lines. The indicated cell lines were infected at an MOI of
FIG. 5.[E1and Methods. Identical amounts of all proteins derived from the in-fections were extracted, electrophoretically separated, and visualizedby either Coomassie staining (A) or autoradiography (B) of the gels.The locations of the hexon, 100K, penton,
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