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The Vaccinia Virus 14-Kilodalton (A27L) Fusion Protein Forms a Triple Coiled-Coil Structure and Interacts with the 21-Kilodalton (A17L) Virus Membrane Protein through a C-Terminal α-Helix

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Figure

FIG. 2. CD spectra of different mutant forms of the 14-kDa protein. (A) Far-UV CD spectrum of the 14K-A mutant form of the 14-kDa protein
FIG. 3. Helical wheel analysis of the predicted coiled-coil region (residues 44to 72) by the algorithm of Wolf et al
FIG. 4. Sedimentation equilibrium analysis of the 14-kDa protein. (A) Sedimentation equilibrium profile of the 14K-A protein at a concentration of 20 �mM Tris-HCl (pH 7.4) buffer at 25,000function corresponding to a single species at sedimentation equilibri
TABLE 1. Effect of protein concentration on Mw at thesedimentation equilibrium
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