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Nuclear import of SAMHD1 is mediated by a classical karyopherin α/β1 dependent pathway and confers sensitivity to VpxMAC induced ubiquitination and proteasomal degradation

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Academic year: 2020

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Figure 1 Determination of the SAMHD1 nuclear localization signal (NLS). A) Comparison of the SAMHD1 amino acid sequence with knownnuclear localization signals (NLS) from EBNA1, polyoma virus large T antigen, adenovirus E1a and human EXO1 identifies an N-te
Figure 2 SAMHD1 nuclear import is mediated by KPNA2 and KPNB1. A) HeLa cells stably transduced with an MLV-vector encoding YFP-taggedwild type SAMHD1 were transiently transduced with Retro-SIREN-Q (Clontech) shRNA encoding vectors expressing two independen
Figure 3 SAMHD1WT and SAMHD1K11A bind VpxMAC with similarefficiencies. 293 T cells were co-transfected with plasmids expressingGST, GST-SAMHD1 or GST-SAMHD1K11A along with plasmids expressingFlag-tagged Vpx derived from HIV-2ROD, SIVMAC or SIVRCM
Figure 4 SAMHD1K11A has reduced sensitivity to VpxMAC mediated degradation. A) 293 T cells were transfected with plasmids encoding YFP,YFP-SAMHD1WT, or YFP-SAMHD1K11A along with plasmids encoding for Flag-tagged Vpx proteins from HIV-2ROD, SIVMAC or SIVRCM
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