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Structural Features of a Picornavirus Polymerase Involved in the Polyadenylation of Viral RNA

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Figure

FIG 1 Picornavirus RNA-dependent RNA polymerases. (A) Diagram of a poliovirus/rhinovirus RNA genome; (B) structure of the poliovirus RNA-dependentRNA polymerase (3Dpol) in the apo form (13); (C) the structurally homologous rhinovirus RNA-dependent RNA poly
TABLE 1 Impact of 3Dpol mutations on virus growth and polyadenylation
FIG 2 Impact of 3D2 mM guanidine HCl (GuHCl), an inhibitor of viral RNA synthesis, as previously described (resis in 1% agarose gels and detected by phosphorimaging
FIG 4 Impact of tyrosine mutations in the fingers domain of 3Dto analyze the quantitative distribution of poly(A) tails in virion RNAs
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