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Cyclin D1 cooperates with p21 to regulate TGFβ mediated breast cancer cell migration and tumor local invasion

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Figure

Figure 1 TGFb induces cyclin D1 expression in highly migratory breast cancer cells. (A) MDA and SCP2 cells were stimulated with orwithout 5 ng/ml transforming growth factor beta (TGFb) for the indicated times
Figure 2 TGFDAPI (blue). The scale bar is 10 μm.between p21 and cyclin D1 is represented by overlay.analyzed by co-immunoprecipitation using an anti-cyclin D1 antibody
Figure 3 Cyclin D1 is required for TGFb-mediated cell migration. (A) SCP2 cells were transfected with Scr or cyclin D1 siRNAs and thentreated with or without 5 ng/ml transforming growth factor beta(TGFb) for 24 hours
Figure 4 Cyclin D1 is a downstream mediator in TGFthe top of growth factor-reduced Matrigel and then treated with or without 5 ng/ml TGFcyclin D1 siRNA-transfected SCP2 cells were treated with or without 5 ng/ml transforming growth factor beta (TGFImmunocy
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