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Overexpression of Promyelocytic Leukemia Protein Precludes the Dispersal of ND10 Structures and Has No Effect on Accumulation of Infectious Herpes Simplex Virus 1 or Its Proteins

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Figure

FIG. 1. PML cloned in a plasmid or baculovirus is expressed and modified by SUMO-I. (A and B) HEp-2 cells were transfected with pAcCMV(lanes 4 and 8) or pAcCMV-PML (lanes 1, 2, 5, and 6) or were untransfected (lanes 3 and 7)
FIG. 2. Overexpression of PML results in the formation of large nuclear structures (ND10) containing CBP and Daxx
FIG. 3. Overexpressed PML remains associated with enlarged ND10 structures but does not affect the localization of ICP0 in the course ofinfection of pHF fibroblasts
FIG. 4. ICP0 colocalizes, but does not overlap, with PML. pHF fibroblast slide cultures were infected with Bac-PML and HSV-1(F) at 3 h afterHSV-1 infection and were treated as indicated in the legend for Fig
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