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In Vitro Synthesis of Turnip Yellow Mosaic Virus Coat Protein in a Wheat Germ Cell-Free System

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Figure

FIG.1.variouscentrationsweretion. Dependence on TYMV RNA concentra- The reactions were performed with optimal con- ofMg2+ (3.3 mM) and K+ (140 mM) and TYMV RNA concentrations
Fig. 6.coatconcentration In the absence of spermine, the Mg2+ yielding maximum synthesis of protein also yields maximum incorpora-
FIG. 3.RNAacid.synthesis(molthe68,000),incubationpg Analysis by SDS-polyacrylamide gel electrophoresis of in vitro synthesized products
FIG. 5.andcomigrating(a)wells Immunodiffusion test. The reaction was performed as described in Materials and Methods

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