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Figure

Figure 1Purification and cleavage of Trx-Y87G2A.14 fusionµkDa), ovalbumin (45 kDa), glyceraldehyde 3-phosphate dehy-drogenase (36 kDa), carbonic anhydrase (29 kDa), trypsino-gen (24 kDa), soybean trypsin inhibitor (20 kDa) and lane 3, 3 lactalbumin (14.2 k
Figure 2Identification of reaction products of CoA hydrolysis.Reaction mixtures containing 0.5 mM CoA were incubated at37°C for 20 min with or without 0.1 µg Trx-Y87G2A.14fusion protein and the products separated by HPLC asdescribed in Materials and method
Table 1: Kinetic parameters for the hydrolysis of CoA and CoA derivatives by Trx-Y87G2A.14 fusion protein
Figure 5Partial sequence alignment of Y87G2A.14 and related sequences.AK009700; At5g45940, BAB09322; YDH5, Q92350; DR1184, Q9RV46

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