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The E6/E7 promoter of human papillomavirus type 16 is activated in the absence of E2 proteins by a sequence-aberrant Sp1 distal element.

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Academic year: 2019

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FIG.1.50thedocumentedE2-binding bp Sequence comparison between the TATA box of the E6/E7 promoter (shaded) and a second AT-rich region located approximately upstream of six different HPVs
FIG. 3.extractoligonucleotideandpromotertheoligonucleotides,ComplexIncubation Bandshift experiment with radiolabeled oligonucleotides comprising either the fp2u site of HPV-16 or an Spl site of SV40
FIG. 5.tractsbinding(ct)comingtranscriptionRNasepromotertranscriptsinitiatedquencestionsequences; Effects of in vitro transcription with SiHa nuclear ex- on HPV-16 P97 promoter activity independence of an Spl- site
FIG. 6.TATAfromenhancer6,andP97P97start 9 In vivo transcription from wild-type and mutant HPV-16 P97 promoters after transient transfection into HeLa (a and d), CaSki (b), SiHa (c) cells

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