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DNA replication roadblocks caused by Cascade Interference complexes are alleviated by RecG DNA repair helicase.

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Figure

Figure 1. E. colipurified within the Cascade complex (500 nM). (B). Agarose gel (0.8%) showing R-loop plasmid mobility shifts when Cascade-crRNA1 was titrated into pUC18 thatmigrated as supercoiled and nicked DNA as indicated (50 ng)
Figure 2. IcrRNA. Replication reactions on nicked plasmid DNA are initiated from a flapengineered into M13 and proceed by rolling circle replication, as illustrated inthe cartoon left
Figure 4. RecG (25, 50 and 100 nM) displaces Cascade R-loops from plasmidof DNA replication

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