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Mapping of Class II Promoter Sites Utilized In Vitro by T7-Specific RNA Polymerase on Bacteriophage T7 DNA

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Figure

FIG. 1.partiallygestedBondigestedrestriction preparation; a Restriction analysis of Bgl II fragment B 0.7% agarose gel
FIG. 2.details).from Sedimentation of Bgl II-cleaved T7 DNA on the first 10 to 30%0 sucrose gradient
FIG. 3.cleavedDNA2.0%T7-specific Electrophoretic analysis of the transcripts made by T7-specific RNA polymerase from Bgl II- T7 DNA and Bgl II fragment B
Table 2, we position the four class II promotersVII to X at 13.5, 18.9, 22.6, and 27.9%, respec-tively, on the standard T7 map (Fig
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