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LFA-1 Expression on Target Cells Promotes Human Immunodeficiency Virus Type 1 Infection and Transmission

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FIG. 1. Virus DNA detected in J�cells. Positive controls (cluded in each experiment. (B) In a separate experiment, HIV-1 GagDNA was amplified from diluted lysates of IIIB-infected JAn HIV-1 Gag control (JThese viruses were originally produced in JPCR and So
FIG. 3. Virus replication in J�type, mutant, or no LFA-1. Jand p24 production in 22.7 transfectants expressing wild-�2.7 cells were infected with SF33 or IIIB, � 106 cells was measured by enzyme-linkedimmunosorbent assay over time.
FIG. 4. Number of infected cells in J�and JInfected cells were detected by intracellular p24 staining and quanti-2.7/mock, J�2.7/LFA-1 wt,�2.7/LFA-1 � cultures following infection with SF33 or IIIB.tated by flow cytometry.

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