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A large deletion in the matrix domain of the human immunodeficiency virus gag gene redirects virus particle assembly from the plasma membrane to the endoplasmic reticulum.

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Figure

FIG. 1.box.risedifferent Schematic representation of the AMA deletion. At the top of the figure, the coding region of the HIV-1 genome is shown, with the open reading frames depicted as open boxes
FIG. 2.Western4)sientharvestedonCA.transfectioncellaredesignated11 and and were Western blot analysis of gag gene products after tran- transfection
FIG. 3.products.preimmuneplexesbandsAMAfectedafter(lanesPEGCAcorrespondanti-gp120respectively.(inare Immunoprecipitation of radiolabeled HIV-1 env gene COS 7 cells transfected with pNL4-3 (WT) or pNLA3- (AMA) were labeled with [3H]glucosamine from 12 to 48
FIG. 4.virus.cles.concentrationsRAU/mlsubjectedansynthesized100 Infectivity of wild-type (pNL4-3) and mutant (AMA) MT-4 cells were infected with equal amounts of virus parti- At the times indicated, cleared media were analyzed for newly Gag antigen by ELIS
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