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The Specificity of TRIM5α-Mediated Restriction Is Influenced by Its Coiled-Coil Domain

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Figure

FIG. 1. The TRIM5�and either human wild-type TRIM5(C) MDTF cell lines expressing the indicated TRIM5B-Box2, coiled-coil, and PRYSPRY) with the linker regions 1 and 2 (L1 and L2) are depicted
FIG. 2. The coiled-coil domain can condition TRIM5�human TRIM5SIVmac, HIV2-, or HIV1-based GFP vectors initially titered on parental MDTF cells
FIG. 4. Positions 186, 214, and 229 of the CC domain condition TRIM5�encompassing positions 168 to 236 derived from squirrel monkey (sq) and human (hu) TRIM5by site-directed mutagenesis in gray
FIG. 6. The squirrel monkey coiled-coil domain can efficiently me-diate multimerization
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