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ERβ1 represses basal like breast cancer epithelial to mesenchymal transition by destabilizing EGFR

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Figure

Figure 1 ERMDA-MB-231 cells following incubation with EtOH or 17b1 inhibits invasion and migration in breast cancer cells by regulating EMT
Figure 2 ERmiR-200a-b-429-regulated reporter assay. The graphs show the mean of three separate experiments with SEM andindicated).MDA-MB-231 cells were transfected with inhibitors of miR-200a, miR-200b and miR-429 or a negative control inhibitor
Figure 3 EGFR promotes EMT and its down-regulation is involved in ERempty pBABE vector (ERexpression by immunoblotting (scale bars, 50ERseparate experiments with SEM andMDA-MB-231 cells following incubation with 5 ng/ml TGF-with the untreated Lenti cells (
Figure 4 ERb1 induces ubiquitylation and degradation of EGFR. (A) Control (Lenti) and ERb1-expressing MDA-MB-231 cells were incubatedin the presence of 100 μM cycloheximide and 10 ng/ml EGF for the indicated times and analyzed for EGFR expression by immuno
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