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Cloning and biochemical characterization of an endo 1,4 β mannanase from the coffee berry borer hypothenemus hampei

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Academic year: 2020

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Figure

Figure 1 Alignment of the deduced amino acid sequence of HhMan of Hypothenemus hampei with those of other GH5 mannanases.Identical or similar amino acid residues are listed in black or gray, respectively
Figure 2 SDS- page, native- page and zymogram of the purified rHhMan. A) SDS-PAGE. M: Broad Range weight marker, C: Negative controlfor expression, I: Unpurified samples
Figure 3 Characterization of recombinant rHhMan. A) The effect of pH on the activity of rHhMan
Figure 5 Michaelis- menten and lineweaver- burk plots of rHhMan using guar gum as substrate

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