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Terminal Nucleotidyl Transferase Activity of RecombinantFlaviviridae RNA-Dependent RNA Polymerases: Implication for Viral RNA Synthesis

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Figure

FIG. 1. HCV NS5B has TNTase activity. (A) SDS-PAGE profile ofdifferent RdRps used in this study
TABLE 1. Effect of HCV RdRp-specific inhibitor (structure shownin inset) on RNA synthesis and TNTase activitya
FIG. 4. Effect of Mn2The layout of the figure is identical to that in panel A. (C) Effects of increasing KCl concentration on RNA synthesis and TNTase activity of H(D) Effects of increasing KCl concentration on RNA synthesis and TNTase activity of Band RNA
FIG. 5. TNTase activity does not require the initiation nucleotide.(A) RNA synthesis (lanes 1 and 2) and TNTase (lanes 3 to 12) assays
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