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Herpes Simplex Virus Type 1 Capsids Transit by the trans-Golgi Network, Where Viral Glycoproteins Accumulate Independently of Capsid Egress

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FIG. 2. Transport of the control VSV G tsdifferent temperatures. 143B cells were transfected with GFP-taggedVSV G24 h and examined immediately by immunofluorescence (A) or fol-lowing a further 2 h at 20°C (B) or 31°C (D)
FIG. 3. Viral kinetics. 143B cells were infected with HSV-1 V701 for 7 h at the nonpermissive temperature (A to F)
FIG. 4. Titration of extracellular virus with Vero cells. 143B cellswere incubated with HSV-1 V701 for 7 h at 39°C and then at 20°C for
FIG. 5. Colocalization of capsids with the TGN. 143B cells were infected with wild-type K26GFP virus (in green), incubated for 7 h at 37°C,and chased at 20°C for 6 h
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