X4 Human Immunodeficiency Virus Type 1 gp120 Down-Modulates Expression and Immunogenicity of Codelivered Antigens

We transfected equal amounts of mutant and wild-type NC proviral plasmid DNA along with the pHCMV-g plasmid into 293T cells to produce the viruses; when VSV-G DNA was measured, we

We hypothesize that vaccination with gp120 expressing ␣ -gal epitopes (gp120 ␣ gal ) results in in vivo formation of immune complexes with anti-Gal, which targets vaccines for

The immunogenicity of a plasmid DNA expression vector encoding both Gag and envelope (Env), which produced human immunodeficiency virus (HIV) type 1 virus-like particles (VLP),

In order to assess the quaternary structure of gp120, a recombinant form of this protein lacking the gp41 domain was expressed in mammalian cells by using a vaccinia virus

Since gp120 Da HX1 did not appear to bind CD4 and could inhibit the binding of several CXCR4 ligands, we next assessed whether the mutant gp120 protein bound to the surface of

BALB/c mice were intramuscularly immunized with 100 m g of vector DNA (not encoding a protein), NP DNA, or NPmut DNA, and their spleen cells were restimulated in vitro for 7 days

However, as reported for HIV-1 (29), it is possible that inhibition of CD4-gp120 binding was not observed because the C3 region of HIV-2 gp120 was poorly accessible from the surface

COS-1 cells were transfected with 10 ,ug of pSVIIIenv DNA expressing wild-type or mutant HIV-1 envelope glycoproteins of the HXBc2 strain (28, 59). Sixty hours after transfection,