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Distinct p53, p53:LANA, and LANA Complexes in Kaposi's Sarcoma-Associated Herpesvirus Lymphomas

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Figure

FIG. 1. Analysis of LANA complexes after purification with a sizing column (Sepharose 6B) and Heparin FF column
FIG. 2. Analysis of LANA binding complexes after heparin affinitychromatography. (A) Profiles of the eluted peaks, using wash buffer
FIG. 4. Analysis of the binding activity of p53 upon p53 response elements (RE). Samples were incubated with an immobilized RE oligonu-cleotide, and washed and bound p53 was detected by ELISA
FIG. 6. Nutlin-3a disrupts LANA:hdm2:p53 complexes in vivoin vitroLANA levels and complexes were determined by Western blotting(input) and immunoprecipitation followed by Western blotting
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