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Image analysis in light sheet fluorescence microscopy images of transgenic zebrafish vascular development

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Figure

Fig. 1. (A) A variety of transgenic fluorescent reporter lines, such as tg(kdrl:HRAS-mCherry)s916 [22, 23], can be used to visualize the zebrafish cardiovascular system.(B) Vasculature-specific transgenic lines visualize endothelial cells, which encompassthe vascular lumen, rather than the blood as is the case in microangiography.
Fig. 2. Dorsal cranial volume was measured in the region indicated by white outlines.(A) The dorsal aorta (DA) was chosen as the most ventral boundary, while the dor-sal longitudinal vein (DLV) constituted the most dorsal
Fig. 3. The CNR in the basal artery was found to differ significantly between trans-genic reporter lines, which can be used to visualize different subcellular components ofvascular endothelial cells such as (1) membrane, (2) cytosol, (3) filamenteous actin (n= 6 embryos).
Fig. 5. (A) Correlation between first and last stack in 10 min time-lapse movies wassignificantly increased via applied motion correction
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