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Mutation of the aspartic acid residues of the GDD sequence motif of poliovirus RNA-dependent RNA polymerase results in enzymes with altered metal ion requirements for activity.

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Academic year: 2019

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Figure

TABLE 1. DNA oligomers used to create mutations in 3Dpol gene
FIG. 1. Expression of 3Dpolgenome from nucleotide 5240 to 7369 was subcloned into expression plasmid p in E
TABLE 2. Virus recovery after transfection in the presence ofmetal ions of poliovirus containing 3D-N-329 mutation

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