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A "humanized" green fluorescent protein cDNA adapted for high-level expression in mammalian cells.

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FIG. 2. Restriction maps of the AAV and Ad vector plasmids. Only those restriction sites relevant for the construction of the rAAV plasmids are shown
FIG. 3. FACS analysis of 293 cells transfected with the pTRrecombinant plasmids. 293 cells (six-well dish) were transfected with a total of 2.8�g of DNA, consisting of different ratios ofsalmon sperm carrier DNA, using the conventional calcium phosphate tr
FIG. 5. Fluorescence of G418-resistant clones containing rAAV-GFPpostinfection, cells were split and plated at a low (less than 10%) confluency; 18 h later, G418 was added at a final concentration of 200H2 provirus
FIG. 7. Fluorescence of the blue His-66 mutant of GFPcotransfected with pTRMethods and photographed 4 days posttransfection in a fluorescence micro-H
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