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Effect of Interaction between Hepatitis C Virus NS5A and NS5B on Hepatitis C Virus RNA Replication with the Hepatitis C Virus Replicon

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Figure

TABLE 1. Sequences of primers used in the present study
FIG. 1. Interaction between NS5A and NS5B of the isolate HCV M1LE and the regions essential for this interaction
FIG. 2. (A) Schematic presentation of HCV mutant replicons used in this study. Wild-type M1LE contains the HCV M1LE wild-type sequence.M1LE/5Adel-1, M1LE/5Adel-2, and M1LE/5Adel-3 encode internal deletions of NS5A missing aa 94 to 162 (region 1), aa 277 to 334 (region 2),
FIG. 3. Efficient translation of mutant FLAG-NS5As. COS1 cellswere transiently transfected with mammalian expression vectors ex-
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