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RAC1 GTPase plays an important role in γ irradiation induced G2/M checkpoint activation

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Figure

Figure 1 IR induces Gthe indicated hours, and analyzed for DNA content. Results depict the percentage of cells withexposed to 5-Gy IR, incubated for the indicated hours, and analyzed for cell cycle by fluorescence-activated cell sorting (FACS)
Figure 2 Rac1 inhibition attenuates IR-induced G8 or 24 hours and analyzed for DNA content
Figure 3 Rac1 inhibition abrogates IR-induced Gphosphorylation, as described in Materials and methods
Figure 4 Rac1 inhibition abolishes IR-induced activation ofNSC23766, incubated for 1 hour, and analyzed for Chk1 and Chk2both ATM and ATR signaling
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