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Towards standardization of next generation sequencing of FFPE samples for clinical oncology: intrinsic obstacles and possible solutions

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Figure

Fig. 1 The description of potential artifacts detected in FFPE samples. malization library concentrations were low for all four samples in question
Fig. 2 The mutations counts correlate with the pre-normalization library concentrations
Table 1 Identified exonic mutations in non-EGFR genes
Fig. 3 The counts of mutations detected in various ranges of allele frequencies. Samples with pre-normalization library concentrations as low as 1.5 ng/μl were shown to yield substantial amounts of presumably artifactual variants with high prevalence in th

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