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The binding affinity of PTPN13’s tandem PDZ2/3 domain is allosterically modulated

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Academic year: 2019

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Figure

Fig. 1 Superposition of 2Dthetitration with PRK2 at various molar ratios up to 1:10 recorded at700 MHz proton frequency at 298 K and pH 7.4 [Black (ligand free), 1H-15N-HSQC NMR spectra recorded on 15N-enriched single PDZ2 domain (12 kDa) recorded at 600 M
Fig. 2 a Superposition of a representative region of the 2D 1H-15N-HSQC NMR spectra of PDZ2 in the absence (red) and presence of PDZ3 (blue).b Only very minor chemical shift differences could be observed, if at all
Fig. 4 After the titration of the PDZ2 and PDZ2/3 tandem domains with the APC peptide, repectively, NMR amide backbone resonances of PDZ2 differ innot congruent anymore
Table 1 Dissociation constants (Ksingle PDZ2 and PDZ3 [D) of APC and PRK2 for the12] domains as well as for the PDZ2/3tandem domain of PTPN13
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