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Mutational analysis of vaccinia virus nucleoside triphosphate phosphohydrolase II, a DExH box RNA helicase.

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Figure

FIG. 1. Nickel affinity chromatography of His-NPH-II. The soluble lysatefrom coinfected BSC40 cells was chromatographed on Ni-nitrilotriacetic acid-
FIG. 2. Phosphocellulose chromatography of His-NPH-II. Phosphocellulosechromatography was performed as described in Materials and Methods
FIG. 5. RNA binding assays of His-NPH-II-Ala mutant proteins sedimentedthrough a glycerol gradient
FIG. 7. ATP hydrolysis by wild-type and mutant His-NPH-II proteins as afunction of enzyme concentration
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