• No results found

Biochemical Analyses of the Interactions between Human Immunodeficiency Virus Type 1 Vpr and p6Gag

N/A
N/A
Protected

Academic year: 2019

Share "Biochemical Analyses of the Interactions between Human Immunodeficiency Virus Type 1 Vpr and p6Gag"

Copied!
6
0
0

Loading.... (view fulltext now)

Full text

Loading

Figure

FIG. 1. Purification of recombinant p6 and Vpr1–71(lane 4), and VprSDS-PAGE and Coomassie blue staining of GST-p6 following gluta-thione-Sepharose affinity chromatography (lane 1), GST-p6 aftercleavage with thrombin (lane 2), p6 after purification over S Seph
FIG. 4. Cross-linking of Vpr1–71. Vpr1–71 at 50 �M was incubatedwith the indicated molar ratios of EGS for 60 min at 37°C and visu-
FIG. 5. Biosensor analysis of p6 binding to Vpr1–97 and Vpr1–71.Representative sensorgrams of p6 injected at concentrations of 0, 7.5,
TABLE 1. Estimated dissociation constants for HIV-1Vpr-p6 complexes

References

Related documents

The concept of FDDS was described in the literature as early as 1962. Floating drug delivery systems is one of the important approaches to achieve gastric retention to obtain

Unexpectedly, generation of 3AB(wt) in trans was not able to efficiently complement the defective replication complex; however, cotranslation of the large P3(wt) precursor

This is to certify that this dissertation titled, “ NEOADJUVANT CHEMORADIATION IN LOCALLY ADVANCED RECTAL CANCER” is a bonafide record of the work

Jan, R.A.et.al worked on 450 patients from September 1997-March 2000 for any neurological complications after popliteal nerve block for foot/ ankle surgery using posterior

Tumors such as renal cell carcinoma, lymphoma, sarcomas, neuroendocrine tumors and angiomyolipoma, which usually are found in adult kidneys, occur rarely in children but do not

Given that RNA g -phosphate cleavage by the wild-type enzyme is 100 times faster than RNA cap formation (27), it is likely that a 1% residual triphosphatase activity suffices

Quantitative analysis of primer extension experiments with RNA from KG cells grown in monolayer and raft culture showed a strong induction of this transcript in differentiated KG

This complex was present in much smaller amounts when the probe was incubated in the presence of the translated mosquito La protein (as shown in Fig. 4) than when incubated with the