Initiation of poliovirus plus-strand RNA synthesis in a membrane complex of infected HeLa cells.

Based on the above and previous data (17), we propose that RIII( ⫺ ) replication enhancer in tombusviruses can facilitate plus-strand RNA synthesis by (i) binding to the replicase,

The data indicated that the first element facilitates plus-strand RNA primer translocation or subsequent elongation during plus-strand RC DNA synthesis, while the last two

Each was cloned into a poliovirus-luciferase replicon cDNA from which RNA could be transcribed in vitro and transfected into HeLa cells (Fig. The kinetics and levels of

BioMed CentralVirology Journal ss Open AcceResearch Stimulation of poliovirus RNA synthesis and virus maturation in a HeLa cell free in vitro translation RNA replication system by

Poliovirus infection at either 37 or 40°C resulted in the accumulation of labeled poliovirus proteins in HeLa cells and the inhibition of host cell protein synthesis (Fig..

This result contrasts with the mode of action of other inhibitors of poliovirus RNA synthesis, such as guanidine or flavones, that selectively block plus-stranded RNA synthesis

The Na+ content of poliovirus-infected HeLa S3 cells increased during the late phase of virus replication, after virus inhibition of host cell protein synthesis and in coincidence

A crude replication complex has been isolated from poliovirus-infected HeLa cells and used for synthesis of poliovirus replicative intermediate (RI) RNA, replicative form (RF) RNA,