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Syntaxin 5-Dependent Retrograde Transport to the trans-Golgi Network Is Required for Adeno-Associated Virus Transduction

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Figure

FIG 1 Inhibition of AAV2 transduction by Golgi apparatus-targeting drugs. (A) HeLa or HEK293T cells pretreated with various concentrations of brefeldin A orgolgicide A were infected with AAV2-Luc at an MOI of 10,000
FIG 2 Lack of evidence for AAV ER transport. (A) HeLa cells transfected withthe ER marker plasmid pER-dTmT (red) were infected with AAV2-Luc at anMOI of 10,000 and fixed at various times postinfection
FIG 3 AAV transduction is independent of retromer function. (A) Validation of siRNA-mediated knockdown of Vps35 by immunoblotting
FIG 4 AAV transduction is independent of late endosome function. (A) Validation of Rab9 knockdown by multiple siRNAs
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