Analysis of the In Vitro Product of an RNA-Dependent RNA Polymerase Isolated from Influenza Virus-Infected Cells

Recombinant bovine viral diarrhea virus (BVDV) nonstructural protein 5B (NS5B) produced in insect cells has been shown to possess an RNA-dependent RNA polymerase (RdRp) activity..

Minimal templates directing accurate initiation of subgenomic RNA synthesis in vitro by the brome mosaic virus RNA-dependent RNA polymerase. Bromovirus RNA replication

The 39kE/CFS template was transcribed by using purified RNA polymerase (lanes 8 and 10) or with extracts prepared during the early phase of viral infection (lanes 9 and 11) and

Viral RNA was synthesized in vitro at the permissive temperature (30 8 C [S 30]) or the restrictive temperature (37 8 C [S37]) after reconstitution of polymerase in vivo at

Antibody to the poliovirus genome-linked protein, VPg, specifically immunoprecipitated the product RNA synthesized in vitro by the poliovirus RNA polymerase and HeLa cell host

For analysis of the products of the in vitro reaction, a postnuclear pellet was prepared and was used as the source of the enzyme and endogenous template.. Samples

The RNA transcripts synthesized in vitro by the intracellular transcriptase complex of VSV- infected cells are copied from virtually all re- gions of the infectious virus genome at

Translation and identification of the mRNA species synthesized in vitro by the virion associated RNA polymerase of vesicular stomatitis virus. Nucleotide sequence of a viral