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Cleavage and Activation of the Severe Acute Respiratory Syndrome Coronavirus Spike Protein by Human Airway Trypsin-Like Protease

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Academic year: 2019

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Figure

FIG. 1. HAT cleaves SARS-S. (A) Cleavage of SARS-S by HAT in cotransfected 293T cells
FIG. 3. Expression of HAT does not induce SARS-S shedding.VLPs were produced in 293T cells by coexpression of HIV p55-Gag
FIG. 4. HAT activates SARS-S for cell-cell fusion in ciscotransfected with pGAL4-VP16 expression plasmid, SARS-S plasmid, and plasmids encoding the indicated proteases or empty plasmid (pcDNA)and mixed with target cells cotransfected with ACE2 expression p
FIG. 5. Activation of SARS-S by HAT does not bypass the requirement for cathepsin activity for virus-cell fusion
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