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Engineering of homologous recombination hotspots with AU-rich sequences in brome mosaic virus.

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Figure

FIG. 1. Schematic representation of the 3�and DIC-Rwhile the corresponding region in DIC-Rupstream RNA1-derived region (positions 163 to 236 in wt RNA1)
FIG. 2. Diagram summarizing the recombination frequencies and distribution of crossover sites in the homologous RNA2-RNA3 recombinants isolated frominfections with wt RNA1, DIC series of RNA2, and PN series of RNA3 constructs
FIG. 5. Incidence of recombination and distribution of crossover sites in homologous recombinant RNA3 molecules obtained with pairs of RNA2-RNA3 constructshaving heterologous downstream sequences with average or high A/U content
FIG. 7. Analysis of accumulation levels of various parental RNA2 mutantsand reconstructed RNA3 recombinants in whole plants, as determined by North-

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