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Dominant-negative synthesis suppression of voltage-gated calcium channel Cav2.2 induced by truncated constructs

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Figure 1.Functional expression of GFP-tagged Cawith: GFP–CapanelmV. Test potentialsmV shown).GFP–CapA/pF (Cav2.2 and formation offunctional channels by coexpression of constructs consisting of Dom I–IIand Dom III–IV
Figure 2.Expression of GFP-tagged Caand Dom I–II (Dom III–IV. Cells were transfected with: GFP–Cav2.2 together with Dom I–II orv2.2 (a), GFP–Cav2.2b), GFP–Cav2.2 (c), GFP–Cav2.2 and Dom I–II (d);GFP–Dom I–II (e); Cav2.2 and GFP–Dom I–II (f); and GFP–Cav2.2
Figure 3.Functional expression of GFP-tagged Caas for Figure 1 because the experiments were all performed in parallel.GFP–Ca0.8 mV;�cDNA constructs stated.v2.2 together with constructs consisting of Dom I–II or Dom III–IV
Figure 4.Properties of single channels formed by Ca�Dom I–II. Recordings were obtained from cell-attached patches from cells transfected with the statedv2.2 compared with coexpression of Dom I–II and Dom III–IV, and coexpression of Cav2.2 with �1 subunit o
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