Detection of sex specific proteins in chick embryo gonads and mesonephros: effects of estradiol benzoate or tamoxifen on their expression

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Figure

Fig. 1. Gonadalproteinpatternsin normalchick embryos.Two-dimensionalgel electrophoresis of cytosol (a, bl and membrane Ie, dl fractions ofthegonadsfrom malela
Fig. 1. Gonadalproteinpatternsin normalchick embryos.Two-dimensionalgel electrophoresis of cytosol (a, bl and membrane Ie, dl fractions ofthegonadsfrom malela p.3
Fig. 2. Mesonephricproteinpatternsin normalchick embryos.Two-dImensionalgel electrophoresisof cytoso/la,bl and membraneIe
Fig. 2. Mesonephricproteinpatternsin normalchick embryos.Two-dImensionalgel electrophoresisof cytoso/la,bl and membraneIe p.4
Fig. 3. Cuts of gels from 19 day old normalembryonicpolypeptides T1, T2, T3. T4 (see Fig_femaleand male chick gonadsand mesonephros.a 1, b"C1 depict spots 01, 02, 03 (seeFig
Fig. 3. Cuts of gels from 19 day old normalembryonicpolypeptides T1, T2, T3. T4 (see Fig_femaleand male chick gonadsand mesonephros.a 1, b"C1 depict spots 01, 02, 03 (seeFig p.5
Fig. 4. Gonadalproteinand membranepatternsin estradiolbenzoateand tamoxifen-treatedchick embryos.Two-dimensionalgel electrophoresisof cytosol (a) leI fractions of the testis from estradiolbenzoate-treatedembryos.and of cytosol (b) and membrane (d) fractions of the ovary fromramoxifen-treatedembryos.Sex-specificpolypeptidesare indicated by arrows.
Fig. 4. Gonadalproteinand membranepatternsin estradiolbenzoateand tamoxifen-treatedchick embryos.Two-dimensionalgel electrophoresisof cytosol (a) leI fractions of the testis from estradiolbenzoate-treatedembryos.and of cytosol (b) and membrane (d) fractions of the ovary fromramoxifen-treatedembryos.Sex-specificpolypeptidesare indicated by arrows. p.6
Fig. 5. Histologicalsurveyof left testisand ovary from a 19 day old chick embryo.(a) controltestis: Ib) testis from estradiol benzoate-treated embryo:note the presenceof a superficialcortex(C) andan underlymgmedulla(M)withtesticular cords; Icl control ovary; (d) ovary from a ramoxifen-treatedembryo: the ovary is reducedin sIze and featuresa thin cortex(C), x96
Fig. 5. Histologicalsurveyof left testisand ovary from a 19 day old chick embryo.(a) controltestis: Ib) testis from estradiol benzoate-treated embryo:note the presenceof a superficialcortex(C) andan underlymgmedulla(M)withtesticular cords; Icl control ovary; (d) ovary from a ramoxifen-treatedembryo: the ovary is reducedin sIze and featuresa thin cortex(C), x96 p.7

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