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Development and organization of glial cells in Drosophila melanogaster

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Figure

Fig. 1. Glial cell organization
Fig. 3. Schematicrepresentationofthelineageof a sensoryorgan.IA)The externalsensoryorgan, which performsmechano-or chemoreceptorfunction,oflginatesfrom rhe sensoryorgan precursorcell (SOP)
Fig. 4. Glial andneuronalpresentparedshowncord displaysorganizationin glideembryos.Mutantembryolabeledwiththe glialmarkerRK2 (A)or withRK2 andthe neuronalmarkeranti-HRP(8)(takenfromVincentet al.,1996)
Fig. 6. Clonalanalysisgenome.usingtheFLPaserecombinase.{AIThe 'FLP out'transposonharboringrhe FLPasetargetsites(FRTs)is inregratedin theFRTs(~)separatetheLaeZgenefrom an Actin5C ubiquitous promoter and hence prevents LaeZ transcription Sequences betweenFRTscontaina transcriptionterminationsitewhicheliminatesthe possibilityof readrhroughof the polymerase.The LacZ gene carries sequencesrhatlocalizethe f5-galproduct in the nucleus (nuc-lacZJ.On another chromosome is integrated a P element carryInga heat shock promoter fused with the FLPasecoding sequences.Heat inducrtonleads to intrachromosomal recombination between the FRTsAs a consequence,LacZ is e\pressedin the celf in whichrecombinationhastakenplaceas well as In Its progeny.(81 Exampleof a clonalanalysisin thewing.A cloneof B-gal expressingcells(red) can be seenin the distal part of a pupalwingTwo glial nuclei(fifled arrows)expressingB-gal and thereforebelongingto the cloneare foundoutsideof the clone,alongtheL 1 nerve(green)(seeemptyarrow).This indicatesthatgl/al cellsoriginatefromwingepithelialceffsandthattheyare ableto migrateduringdevelopment.In this protocol,recombinationis inducedat early stagesof development,at a stageat whichthe cellsthatwill giveriseto thewingareactivelyproliferating.The fate of wing cells IS analyzedat larer stages,whenall the eel/typeshavedifferentiated.(so) indicatescells of the sensoryorgans.Bar, 25p.

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