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Investigation of testosterone, androstenone, and estradiol metabolism in HepG2 cells and primary culture pig hepatocytes and their effects on 17βHSD7 gene expression

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Table 1. Real-time PCR primer sequences for amplification of 17bHSD7 gene, and for housekeeping gene hypoxanthinephosphoribosyltransferase (HPRT).
Figure 1. Effect of androstenone or androstenone in combination with10 nMIncubation time was 24 hr
Figure 3. Chromatogram and mass spectra of androstenone and metabolite. The (A) represents androstenone in the medium before cellculture, and the (B) represents identified androstenone metabolite in the medium after cell culture.doi:10.1371/journal.pone.0052255.g003
Figure 5. Chromatogram and mass spectra of band the (B) represents identified-estradiol and metabolite

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