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Functional CD40 ligand is expressed by T cells in rheumatoid arthritis

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Academic year: 2020

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Figure

Figure 1. CD40-L expression by RA PB and SF T cells and in RA synovial tissue. (A) T cells were prepared from 9 normal PB, 5 RA PB, or 12 RA SF samples by rosetting with neuraminidase-treated SRBC, and stained immediately with either biotinylated control m
Figure 2. CD4 and
Figure 3. Characterization of CD40-L expression using RT-PCR. cDNA derived from freshly purified or in vitro activated normal PBMC, or freshly purified RA PB or SF MNC or T cells was ampli-fied with specific primers for either CD40-L or �-actin
Figure 5. Cycloheximide does not block rapid CD40-L expression by RA SF T cells. RA SF T cells were incubated with or without cyclohexim-ide for 2 h, washed, and then activated with PMA and ionomycin, with or without cycloheximide, for various periods of t
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