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Clathrin and AP 1 regulate apical polarity and lumen formation during C elegans tubulogenesis

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Figure

Fig. 1. Apicobasal polarity alteration and ectopic lumen formation in CHC-1-depleted intestines
Fig. 2. Depletion of four different AP-1 adaptor subunitsphenocopies the polarity and ectopic lumen defect induced byphenotypes of corresponding presumed null alleles (right) showingbasolateral ERM-1::GFP displacement (arrows) and ectopic lumenformation (a
Fig. 3. Placement of polarized transmembranous,submembranous and junction molecules in AP-1-depletedcytoplasmically before being displaced to ectopic lumens (EL): (B) actin(phalloidin), ERM-1 overlay; (C) intermediate filaments (IFB-2); (D) OPT-2; (E) PAR-
Fig. 4. Effect of clathrin/AP-1 depletion on post-Golgi vesicles. Selective decrease of apical endosomal subfractions in **PGFP::RAB-10 (arrows); note the perinuclear GFP::RAB-10 assembly
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