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Identification of Canine Coronavirus Strains from Feces by S Gene Nested PCR and Molecular Characterization of a New Australian Isolate

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Figure

FIG. 1. Comparison of PCR and nPCR amplification of differentcell culture-propagated CCV strains, processed as described in Mate-
TABLE 2. Comparison of virus isolation and nPCRassay as detection methodsa
FIG. 3. Amino acid sequence alignment of nPCR assay S gene product for CCV 1–71 (AF116246), CCV NVSL (AF116244), CCV K378(X77047), CCV 6 (A22882), CCV C54 (A22886), CCV INSAVC (D13096), CCV 5821 (AB017789), CCV TN449 (AF116245), CCV UCD2
FIG. 5. Phylogenic tree of CCV and FoCV S gene sequences basedon DNA parsimony using the PHYLIP package as described in the

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