• No results found

Droplet-based microfluidic high-throughput screening of heterologous enzymes secreted by the yeast Yarrowia lipolytica

N/A
N/A
Protected

Academic year: 2020

Share "Droplet-based microfluidic high-throughput screening of heterologous enzymes secreted by the yeast Yarrowia lipolytica"

Copied!
14
0
0

Loading.... (view fulltext now)

Full text

Loading

Figure

Fig. 2 Hydrolytic activities of crude supernatants. JMY4346 (YNB blank (buffer pH 4.5 containing 150 μM of cellobiose‑based substrate for strains JMY4469 (lineassay performed in 50 mM acetate buffer pH 4.0 containing 25 μg/l of Enzchek Protease substrate f
Fig. 3 Droplet‑based microfluidic screening platform. 1. Single cell encapsulation: yeast cells were encapsulated in 20 pl droplets at 1200 droplets/s
Fig. 4 JMY4346 ( xlnb) and JMY4510 ( xlnc) strains activity in droplets 2D histograms showing the blue fluorescence (xylanase activity) and the red fluorescence (barcoding) of a binary 20 pl droplets emulsion containing JMY4346 (xlnb) and JMY4510 (xlnc) st
Fig. 5 JMY4346 (xlnb), JMY4469 (cbha) and JMY4407 ( pep1) strains activity in droplets 1D histograms showing the number of droplets observed as a function of the enzymatic activity (blue or green fluorescence) for a ternary emulsion containing JMY4346 (xln
+2

References

Related documents

Because of the recommended procedure for evaluating reliability (and validity) is to have bilingual participants fill out the questionnaire in both languages and assess the

The small premature infant who has undergone a sur gical procedure and would have to be fed by gavage is much more easily and safely fed through a gastrostomy tube (Fig. 2)..

19% serve a county. Fourteen per cent of the centers provide service for adjoining states in addition to the states in which they are located; usually these adjoining states have

420 /- (charges are inclusive of services tax) and Services charge of Rs. 350 / respectively, charged by Stock Holding Corporation. Service charges of various Depositary

This server can be a computer on the local network, an access. point / router with integrated authentication server, or a

/ L, and a different adsorbent mass, is shown in Figure 5. The results obtained show that the removal rate increases quickly at first, until a time of 60 min, and