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A dynamic method based on the specific substrate uptake rate to set up a feeding strategy for Pichia pastoris

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Figure

Figure 1 Experimental strategy for the determination ofcultivations. The calculated yields (Yused to set up a feeding profile fortime; qrelevant strain specific parameters in a batch cultivation withmethanol pulses
Figure 2 Batch cultivation with methanol pulses of 0.5, 1 and2% (v/v). A, calculated specific substrate uptake rate qs (circle) andcarbon dioxide evolution rate (CER); B, specific production rate qp(diamond) and carbon dioxide evolution rate (CER).
Table 1 Description of feeding strategies for fed batch cultivations based on the specific substrate uptake rate qs
Figure 3 Specific substrate uptake rate profiles which wereC1 (black triangle down), offline determined qA (black triangle up), offline determined qtriangle up); theoretical qtriangle down); theoretical qused for different fed batch cultivations
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