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Development of a self-replicating plasmid system for Mycoplasma hyopneumoniae

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Figure

Table 1 Oligonucleotide sequences.
Figure 1 oriCindividual clones (C1 and C2) and analysed by agarose gel electrophoresis along with plasmid (P) DNA controland transformantsto produce plasmids pMHO-1, pMHO-2, pMHO-3 and pMHO-4 respectively(shown in bold) and putative TATA box location (unde
Figure 2 Minimum oriC determination. The predicted oriC region of M. hyopneumoniae strain 232 is shown with the location of two putativeDnaA boxes (black arrowheads) lying within AT-rich intergenic regions (grey boxes)
Table 2 Effect of electroporation voltage ontransformation frequency.
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