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Histone H1c decreases markedly in postreplicative stages of chicken spermatogenesis

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Figure

Fig. 1. Polyacrylamidegel electrophoresischickenof histoneH1 proteinsfromreticulocytesla)andchickenerythrocytes(b).HistoneHIproteinswere electrophoresedon an acid/urea18% polyacrylamidegel at150 V for 72 h
Fig. 3. Changes in the relativeproportionsfromof H1 proteinsobtainedchickentestiscellsat successivestagesof spermatogenesis.ChickentestiscellswereseparatedbycenrrifugalelutriationandH 1proteinswereextractedfromeachfractionand electrophoresedin aceticacid/ureapolyacrylamidegels.H1 proteinswerequantifiedfrom gel scansor by dye elutionfromthe excisedbands,as describedin the MaterialsandMethodssection.Theresolutionobtainedpermitsthequanrificationoffour majorbands:H1a (H1a+H1a')(::J), H1b1,O), H1c(H1c+H1c')(8J, andHldl_l.
Fig.4.chickenmaximumresiduesindicatedCarboxy-terminalsequencesofhistoneH1 derivedfromthegenes.The sequencedataarefromthe references(Colesetal.
Fig. 6. Reactionof 5% perchloricacidsolubleproteins,obtainedfromratandchickentissues,withantiserumto ratH1°.HistoneH1 proteinsand other5 % perch/oTicacid solubleproteinsobtainedfrom rat and chicken tissueswere electrophoresedin15%aceticacid/ureapolyacrylam

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