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Analysis of polysulfate binding domains in porcine proacrosin, a putative zona adhesion protein from mammalian spermatozoa

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Figure

Fig. 1. Aminoacid sequenceand Cysof porcineproacrosin(from Adhametal., 1989 and Baba et al., 1989)
Fig. 3. Fragmentation
Fig. 5. Relative binding(%) of0, 1251_ZPGPsand 1251.fucoidan probestonativespermproacrosin(residues1 to399)andbacterialrecombinantfragments(residuesas indicatedl._,125{_ZPGPprobe1251-fucoidan probe.ns, not significant.*P<0,05;uP<O.01.Values aremeanst:SEM.
Fig. 7. Distributionof basicandacidicresiduesin porcineproacrosin.Heightof verticalbar indicatesresidue.Basics:(I ) arginine;(I) lysine;(I)histidine.Acidics;( I) glutamicacid; (I) asparticacid.

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