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A software solution for recording circadian oscillator features in time-lapse live cell microscopy

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Figure

Figure 1 Tracking cell using DP. A. 5 frames extracted from animage sequence showing the results of the preprocessing step(Laplacian of Gaussian filter) and the reduced version of the images.Cells appear as particles consisting of a few pixels
Figure 2 Segmentation of cellprocedure.next, a gradient is computed in the X and Y directions, in orderfinally to compute the potential functionpotential functionshifted in the vertical direction and the potential function wasrecomputed
Figure 3 Quantification of Rev-VNP circadian expression, cell displacement and nucleus size in NIH3T3 fibroblasts using CGE
Figure 4 Circadian fluorescence pattern and cell motility quantification inPeriod length was calculated as time span between two fluorescence peaks of individual cells after the dexamethasone shock: mean = 25.14 h,SD = 1.56 h, n = 50.Reverba-luciferasemoti

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