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EFFICIENT REGENERATION AND AGROBACTERIUM-MEDIATED TRANSFORMATION PROTOCOL FOR RECALCITRANT INDICA RICE (ORYZA SATIVA L.).

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Figure

Fig 1. The effect of (A) KIN: Kinetin, (B) BAP: 6-benzyladenine aminopurine and (C). TDZ: Thiadizuron from 1 to 6 mg/l on the multiple shoot induction from MR219 rice shoot apices after 2 weeks of culture
Fig 3. The effect of different cytokinins from 1 to 6 mg/l on the biochemical changes – (A) total chlorophyll, (B) total soluble sugar, (C) total soluble protein content of in-vitro shoots after 2 weeks of culture
Fig 5. The PCR product of (A) GFP (~678 bp) and (B) GUS (~640 bp) using mGFP and GUSA specific primers
Fig 6. The acclimatization process of in-vitro cultured rice shoots, hygromycin selection and GFP/GUS expression of rice transformants

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